上海路阳生物技术有限公司为客户提供更理想的光源!
021-64195798
153-17565658
热门关键词:B-100AP LUYOR-3415RG
您的位置:首页>>公司与行业>>公司新闻

北京大学利用LUYOR-3415RG进行西瓜研究

作者:上海路阳生物时间:2022-01-04 09:22:45浏览1926 次

信息摘要:

近日北京大学先进农业科学研究所发表文献《Efficient transformation and genome editing of watermelon assisted by genes that encode developmental regulators》,文献中提及利用LUYOR-3415RG便携式荧光蛋白激发光源快速筛选dsred在西瓜上的表达。

北京大学利用LUYOR-3415RG进行西瓜研究

近日北京大学先进农业科学研究所发表文献《Efficient transformation and genome editing of watermelon assisted by genes that encode developmental regulators》,文献中提及利用LUYOR-3415RG便携式荧光蛋白激发光源快速筛选dsred在西瓜上的表达。文献摘要如下:


Establishment of a watermelon transformation system

 To improve the transformation efficiency of watermelon, we first established a fast and simple detection system for watermelon transformation. Both the neomycin phosphotransferase II (nptII) gene, which confers kanamycin (Kan) resistance in transgenic plants, and the hygromycin phosphotransferase II (hptII) gene, which confers hygromycin (Hyg) resistance, have been used as selectable markers in watermelon tissue culture. Although both Kan and Hyg are aminoglycoside antibiotics that act as protein synthesis inhibitors, Hyg causes more severe damage than Kan during watermelon tissue culture (Park et al., 2005), and the time required for tissue culture in Hyg medium is usually longer than required for Kan; thus, nptII was chosen as the selectable marker. In addition, given the high frequency of false positive plants obtained after Kan selection (Park et al., 2005), the DsRed2 fluorescent protein, which can be easily observed using the LUYOR-3415RG hand-held lamp (Luyor Corporation, Shanghai, China), was introduced into the binary vector as another marker to visually identify stable transgenic events (Figure 1a). The reporter vector was named pW501.

QQ图片20220104092217.png

Watermelon transformation

 The genetic transformation was conducted as previously described with slight modifications (Tian et al., 2017). In brief, surface-sterilized watermelon seeds were sown on Murashige and Skoog (MS) solid medium for 3 days in the dark. Then the middle parts of the cotyledons without embryo were cut into 1.5 × 1.5 mm pieces as explants. A. tumefaciens strains harboring the indicated binary vectors were co-cultivated with the cotyledon fragments in the dark for 3 days on MS solid medium containing 1.5 mg/L 6-BA. Then the cotyledon fragments were transferred onto selective induction medium containing 2 mg/L 6-BA, 0.2 mg/L IAA, 50 mg/L Kan, and 100 mg/L Timentin. The regenerated adventitious buds were excised and transferred onto elongation medium containing 0.1 mg/L 6-BA, 0.01 mg/L NAA, 100 mg/L Timentin, and 50 mg/L Kan. Plants with full leaves and stems were transferredto rooting medium containing 1mg/L IBA and 100 mg/L Timentin. Positive transgenic events were detected using the hand-held dual-wavelength fluorescent protein excitation light source LUYOR-3415RG (Luyor Corporation,  Shanghai, China) or Zeiss 357 SteREO Discovery.V20.


文献地址:

doi:https://doi.org/10.1101/2021.11.05.467370 

1573530098248906.jpg

LUYOR-3415RG便携式双波长荧光蛋白激发光源能够快速筛选植物种子、愈伤、叶片的荧光蛋白表达,微信扫描下面二维码免费试用:

华南地区请扫描:

1632701552910943.jpg

华北地区请扫描:


【北京大学利用LUYOR-3415RG进行西瓜研究】链接地址:https://www.luyoruv.com/news/news1/650.html
未经公司同意,严禁转载我站文章,经同意转载的,必须注明出处。
返回列表 本文标签: